微核(micronucleus，簡稱MCN)是真核生物細(xì)胞中的一種異常結(jié)構(gòu)，是由有絲分裂后期喪失著絲粒的染色體片斷游離于細(xì)胞質(zhì)中產(chǎn)生的。細(xì)胞受遺傳毒物作用形成微核，以檢測細(xì)胞中微核的形成來檢測物質(zhì)遺傳毒性的試驗稱為微核試驗。目前，微核試驗已被許多國家及機(jī)構(gòu)納入標(biāo)準(zhǔn)檢測試驗，用于評價新藥、食品添加劑、農(nóng)藥、化妝品、工業(yè)化學(xué)品、環(huán)境污染物等遺傳毒性的檢測、安全性評價和遺傳損害的檢測。

體外流式微核分析，讓該過程變得更加簡單、快捷、可靠，并可獲得更豐富的信息。

流式細(xì)胞術(shù)是檢測微核的新興技術(shù)平臺。相比于傳統(tǒng)的顯微鏡下計數(shù)微核的方法，流式細(xì)胞技術(shù)的檢測優(yōu)勢在于：高速高通量，多細(xì)胞多參數(shù)，高特異性，多信息。

In Vitro MicroFlow Kits 流式細(xì)胞術(shù)體外微核檢測試劑


試劑規(guī)格：

IN VITRO-250/50：Analyze 250 samples in 96-well plates or analyze 50 samples in larger format plates or flasks.
IN VITRO-1,000/200：Analyze 1,000 samples in 96-well plates or analyze 200 samples in larger format plates or flasks.
IN VITRO-2,000/400：Analyze 2,000 samples in 96-well plates or analyze 400 samples in larger format plates or flasks.

試劑運(yùn)輸條件：
Ambient

試劑盒存儲條件：
Freezer (-20 °C)
Refrigerator (4 °C)
Ambient

試劑盒組成：
Incomplete Lysis Solution 1: Lyses cells after staining
Incomplete Lysis Solution 2: Lyses cells after staining
Nucleic Acid Dye A: Stains the DNA of cells undergoing apoptosis or necrosis
Nucleic Acid Dye B: Stains nuclei and micronuclei
RNase Solution: Removes RNA so Nucleic Acid Dye B stains only nuclei and micronuclei
10x Buffer: Used in the lysis solutions

相關(guān)材料的要求：
Cell line and cell culture materials
Freezer (-20 °C) and refrigerator (4 °C)
Centrifuge and other general lab supplies
Flow cytometer capable of 488 nm excitation
Light source (fluorescent preferred)
If using 96-well plates, an 8-channel bridge and aspirator manifold (available from V&P Scientific)

Litron Laboratories 相關(guān)產(chǎn)品：
1、Litron Laboratories 流式細(xì)胞術(shù)檢測試劑 產(chǎn)品目錄    
2、In Vitro MicroFlow Kits 流式細(xì)胞術(shù)體外微核檢測試劑 
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