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epicentre HTFP061 pCC2 Forward Sequencing Primer

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epicentre HTFP061

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出廠價(jià)：
9999

主要規(guī)格：
1 nmole

用　　途：
科研

epicentre HTFP061 pCC2 Forward Sequencing Primer 產(chǎn)品簡(jiǎn)介：北京中北林格供應(yīng)epicentre HTFP061 pCC2 Forward Sequencing Primer，中北林格是epicentre總代理，提供epicentre產(chǎn)品貨號(hào)報(bào)價(jià)查詢。其他包括lucigen總代理，cellscript總代理，Biosearch technologies總代理，epicentre總代理，illumina總代理，ICLLAB總代理，ImmunoReagents總代理。
供應(yīng)商：中北林格
產(chǎn)地：us
發(fā)貨地：北京
10 9 pfu / μg DNA）包裝，并包被在試劑盒中提供的TransforMax?EPI300? 大腸桿菌（圖3）。 The kit uses a strategy of cloning blunt-ended DNA fragments generated by random shearing of the DNA, to produce more complete and unbiased genomic libraries than can be obtained by partial restriction endonuclease digests. Genomic DNA is first sheared into approximately 40-kb fragments. The sheared DNA is end-repaired to generate blunt, 5′-phosphorylated ends and then size-selected by and recovered from a low-melting-point agarose gel. Finally, the size-selected DNA is ligated into the cloning-ready CopyControl pCC1FOS or pCC2FOS Vector, packaged using ultra-high efficiency MaxPlax? Lambda Packaging Extracts (>109 pfu/μg DNA), included in the kit, and plated on the supplied TransforMax? EPI300? E. coli (Fig. 3). 好處說(shuō)明可提供CopyControl的CopyControl pCC1FOS和pCC2FOS載體：線性化，去磷酸化，純化并準(zhǔn)備連接。 CopyControl pCC1FOS and pCC2FOS Vectors are supplied Cloning-Ready: linearized, dephosphorylated, purified, and ready for ligation. 無(wú)需部分限制核酸內(nèi)切酶消化或脈沖場(chǎng)凝膠電泳即可制備用于克隆的基因組DNA。 No need for partial restriction endonuclease digests or pulse field gel electrophoresis to prepare the genomic DNA for cloning. 使用pCC2FOS載體最大化高通量末端序列結(jié)果（圖4）。 Maximize high-throughput end-sequence results using the pCC2FOS Vector (Fig. 4). 克隆可以從單個(gè)拷貝誘導(dǎo)到每個(gè)細(xì)胞多達(dá)50個(gè)拷貝（圖5）。安全地獲得更高的DNA產(chǎn)量，同時(shí)保持單拷貝數(shù)克隆的穩(wěn)定性。 Clones can be induced from single copy up to 50 copies per cell (Fig. 5). Safely obtain higher DNA yields while maintaining the stability of single-copy-number clones. 高效的λ包裝消除了背景和誤報(bào)。 High-efficiency lambda packaging eliminates background and false positives. 比BAC克隆更快更容易。 Faster and easier than BAC cloning. epicentre HTFP061 pCC2 Forward Sequencing Primer 圖例說(shuō)明圖1. CopyControl?矢量地圖。用于CopyControl Fosmid庫(kù)生產(chǎn)的CopyControl pCC1FOS?和pCC2FOS?載體在Eco 72 I（平端）位點(diǎn)線性提供，然后脫磷酸化。該載體已準(zhǔn)備好克隆約40 kb的末端修復(fù)（平末端）基因組DNA。 Figure 1. CopyControl? Vector map. The CopyControl pCC1FOS? and pCC2FOS? Vectors for CopyControl Fosmid library production are supplied linearized at the Eco72 I (blunt) site and then dephosphorylated. The vector is ready for cloning end-repaired (blunt-end) genomic DNA of approximately 40 kb. 圖2. CopyControl?pCC2FOS?矢量引物盒。該載體與pCC1FOS載體的區(qū)別在于新引物盒的工程化，該引物盒消除了浪費(fèi)的源自載體的測(cè)序讀數(shù)，并最大程度地減少了在大腸桿菌基因組上引發(fā)的可能性。 Figure 2. The CopyControl? pCC2FOS? Vector primer cassette. The vector differs from the pCC1FOS Vector by the engineering of a new primer cassette that eliminates wasteful vector-derived sequencing reads and minimizes the potential for priming on the E. coli genome. 圖3（單擊放大）。使用CopyControl?Fosmid庫(kù)生產(chǎn)工具包準(zhǔn)備fosmid庫(kù)的過(guò)程概述。一旦準(zhǔn)備好文庫(kù)，就可以使用EPICENTRE的DirectLysis Fosmid96試劑盒或FosmidMAX?DNA純化試劑盒，以小體積培養(yǎng)單個(gè)克隆，并誘導(dǎo)成多拷貝數(shù)，從而獲得高產(chǎn)率的高純度DNA，用于指紋，測(cè)序等。 Figure 3 (click to enlarge). Overview of the process for preparing a fosmid library using the CopyControl? Fosmid Library Production Kits. Once the library has been prepared, individual clones can be cultured in small volume and induced to multiple-copy number for high yields of high-purity DNA for fingerprinting, sequencing, etc., using EPICENTRE's DirectLysis Fosmid96 kit or FosmidMAX? DNA Purification Kit. 圖4.用pCC2FOS?正向引物在pCC2FOS?克隆上以1 / 48x BigDye?稀釋度獲得的典型測(cè)序結(jié)果。用pCC2FOS反向引物獲得了相似的結(jié)果（數(shù)據(jù)未顯示）。 Figure 4. Typical sequencing results obtained with the pCC2FOS? Forward Primer on a pCC2FOS? clone at 1/48x BigDye? dilution. Similar results were obtained with the pCC2FOS Reverse Primer (data not shown). 圖5.每個(gè)細(xì)胞最多可復(fù)制50個(gè)拷貝的CopyControl?Fosmid克隆，以大大提高DNA產(chǎn)量。從未誘導(dǎo)的（–）和誘導(dǎo)的（+）CopyControl克隆中分離出的fosmid DNA的Hin d III消化物。消化液占樣品總體積的三分之一（8 μl），并通過(guò)瓊脂糖凝膠電泳進(jìn)行分析。M線，千磅梯子。 Figure 5. CopyControl? Fosmid clones can be induced up to 50 copies per cell to greatly increase DNA yield. Hind III digests of fosmid DNA isolated from uninduced (–) and induced (+) CopyControl clones. Digests contained one-third (8 μl) of the total sample volume and were analyzed by agarose gel electrophoresis. Lane M, Kilobase ladder. 對(duì)此產(chǎn)品有興趣，可聯(lián)系中北林格獲取更多詳細(xì)信息。

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